MACCONKEY AGAR
Agar
CAS: 9002-18-0
Molecular Formula: C14H24O9
MACCONKEY AGAR - Names and Identifiers
MACCONKEY AGAR - Physico-chemical Properties
| Molecular Formula | C14H24O9 |
| Molar Mass | 336.33496 |
| Melting Point | 85-95°C |
| Water Solubility | SOLUBLE IN HOT WATER |
| Solubility | Insoluble in cold water, easily soluble in boiling water, slowly soluble in hot water |
| Appearance | White-like powder |
| Color | Brownish yellow |
| Odor | Odorless |
| Merck | 14,184 |
| PH | 6.5-6.8 (1.5% in gel, after autoclaving) |
| Storage Condition | room temp |
| Stability | Stable. Incompatible with strong oxidizing agents. |
| Sensitive | Moisture Sensitive & Hygroscopic |
| MDL | MFCD00081288 |
| Physical and Chemical Properties | WATER-SOLUBLE IN HOT WATER |
| Use | Used for the preparation of bacterial culture medium, also used as a stabilizer of colored substance suspension |
MACCONKEY AGAR - Risk and Safety
| Hazard Symbols | Xn - Harmful |
| Risk Codes | R22 - Harmful if swallowed R36/37/38 - Irritating to eyes, respiratory system and skin. |
| Safety Description | S26 - In case of contact with eyes, rinse immediately with plenty of water and seek medical advice. S36 - Wear suitable protective clothing. S24/25 - Avoid contact with skin and eyes. |
| WGK Germany | 2 |
| RTECS | AW7950000 |
| FLUKA BRAND F CODES | 3 |
| TSCA | Yes |
| HS Code | 13023100 |
| Toxicity | LD50 orally in Rabbit: 11000 mg/kg |
MACCONKEY AGAR - Reference
| Reference Show more | 1. Guo Wenbo, Shen Yuanyuan, Yang Junhua, etc. Isolation and identification of fumonisin-producing strain from maize and its toxin-producing conditions [J]. Journal of Agricultural Sciences of Shanghai, 2017, 033(006):78-84. 2. Huang Mei, Shen Jian-ying, Du Cheng-Cheng, et al. Preliminary Study on antibacterial activity of artemisinin and its derivatives [J]. Chinese Journal of Traditional Chinese Medicine, 2019, 29 (9). 3. Yang Yongchao, Wang Zhongyuan, Yang Xiaozhen, etc. Cloning and functional analysis of ClP5CS gene from watermelon [J]. Journal of Northwest Sci-Tech University of Agriculture and Forestry (Natural Science Edition), 2018, v.46;No.337(10):95-106. 4. Li Xiaoli. Protective effects of extracts from different parts of Toona sinensis on Caenorhabditis elegans under heat stress [D]. Fuyang Normal University, 2020. 5. Xin Wang et al [IF = 9.229]. "Low-Cost, Robust-Responsive Smart Windows with Dynamic Switchable transmission. Acs Appl Mater Inter. 2020;12(13):15695-15702 6. [IF = 6.843] Wenqin Li et al."The antibacterial and antibiofilm activities of mesoporous hollow Fe3O4 nanoparticles in an alternating magnetic field."Biomater Sci-Uk. 2020 Aug;8(16):4492-4507 7. [IF=6.321] Xinyi Wu et al."Red Blood Cell Membrane-Camouflaged Tedizolid Phosphate-Loaded PLGA Nanoparticles for Bacterial-Infection Therapy."Pharmaceutics. 2021 Jan;13(1):99 8. [IF=5.396] Bingbing Song et al."SKN-1 is involved in combination of apple peels and blueberry extracts synergistically protecting against oxidative stress in Caenorhabditis elegans."Food Funct. 2020 Jun;11(6):5409-5419 9. [IF=5.396] Bingbing Song et al."Combination of apple peel and blueberry extracts synergistically induced lifespan extension via DAF-16 in Caenorhabditis elegans."Food Funct. 2020 Jul;11(7):6170-6185 10. [IF=4.451] Bingbing Song et al."Raspberry extract ameliorates oxidative stress in Caenorhabditis elegans via the SKN-1/Nrf2 pathway."J Funct Foods. 2020 Jul;70:103977 11. [IF=10.588] Yingying Zha et al."Size-dependent enhancement on conjugative transfer of antibiotic resistance genes by micro/nanoplastics."J Hazard Mater. 2022 Jun;431:128561 |
MACCONKEY AGAR - Nature
Open Data Verified Data
strip Agar is colorless translucent or white to light yellow, the surface shrinkage, slightly glossy, light, soft and tough, not easy to fold, completely dry, brittle and fragile; powdered Agar is a white or yellowish scaly powder. The Agar was odorless and mild in taste. Insoluble in cold water, but can slowly absorb water, swelling and softening, can absorb more than 20 times the water. It is easily dispersed in boiling water to form a Sol, and the Sol is a neutral reaction. More than 0.1% to 0.6% of the Sol can be converted into gel at 28 to 42 °c, and will not melt below 85 °c. Not involved in human metabolism, no nutritional value.
Last Update：2025-06-10 22:55:16
MACCONKEY AGAR - Preparation Method
Open Data Verified Data
water soaked stone cauliflower, Corynebacterium and other red algae to remove impurities, and then sulfuric acid or acetic acid under certain conditions to soak hydrolysis, and then the hydrolysis liquid is filtered, purified, the product is obtained by cooling and solidifying.
Last Update：2025-06-10 22:55:16
MACCONKEY AGAR - Standard
Authoritative Data Verified Data
This line is derived from the slime leached and dehydrated by the stone cauliflower Gelidium amansii Lamx or several other red algae.
Last Update：2024-01-02 23:10:35
MACCONKEY AGAR - Trait
Authoritative Data Verified Data
- linear Agar is slender strip-like, white to light yellow; Translucent, surface shrinkage, slightly glossy, light, soft and tough, not easy to break; After completely dry, brittle and fragile; no odor, light taste.
- the powder Agar is a fine particle or scale-like powder, colorless to light yellow; It is a colorless irregular polygonal viscous fragment, which is colorless and has a light taste when it is applied in cold water and observed under a microscope.
- This product is dissolved in boiling water, insoluble in cold water, but can expand into colloidal blocks; The aqueous solution shows a neutral reaction.
Last Update：2022-01-01 11:26:06
MACCONKEY AGAR - Introduction
Special agar powder for tissue culture. Microbial medium, plant medium, bacterial medium raw materials, immobilized enzyme carriers, bacterial embedding materials and electrophoresis media.
Last Update：2022-10-16 17:29:46
MACCONKEY AGAR - Application
Open Data Verified Data
as an emulsion stabilizer and thickener, Agar has a strong gelling power, and its gel strength increases when it is used together with dextrin or sucrose. China's provisions for all types of food, according to the production needs of the appropriate use.
Last Update：2025-08-19 16:24:40
MACCONKEY AGAR - Differential diagnosis
Authoritative Data Verified Data
- take about l g of this product, add 65ml of water, boil, stir constantly to dissolve, make up the evaporated water with hot water, and cool to 32~39 * C, that is, it is condensed into a translucent and elastic gel, and re-melted when heated to 85 * C.
- take this product (if it is strip, it should be cut and crushed), dip in 0.02mol/L iodine solution, after a few minutes, dye it brown-black, take it out, add water to impregnate it with gradual purple.
- take this product about 0. lg, add water 20ml, heat to dissolve; Take 4ml, add hydrochloric acid 0.5ml, put on water bath heating for 30 minutes, add sodium hydroxide solution 3ml and Alkaline copper tartrate solution 6 m l, put on water bath heating, that is, a red precipitate was formed.
Last Update：2022-01-01 11:26:07
MACCONKEY AGAR - Exam
Authoritative Data Verified Data
suction capacity
take this product 5. 0g, put in a 100ml measuring cylinder, add water to make 100ml, stir, stand at 25°C for 24 hours, filter in another measuring cylinder through wet glass wool, the total amount of filtrate should not exceed 75ml.
starch
take 0.10g of this product, add 100ml of water, boil and dissolve, let it cool, add 2 drops of Iodine test solution, and do not show blue.
gel
take this product l.O g, put it in a beaker, add 100ml of water, put it on a water bath, heat and dissolve it, let it cool to 50°C, take 5ml of the solution, add 0 .2M o l/L potassium dichromate solution and 3M o l/L hydrochloric acid solution mixed solution (4: 1)2~3 drops, no yellow precipitation.
insoluble in water
take about 1.5g of this product, weigh it accurately, put it in a beaker, add water to make it 20 0ml, boil it, stir it while boiling to completely dissolve the Agar, while hot, filter with constant weight vertical melting glass Crucible No. 3, wash the beaker with hot water for several times, filter, dry the filter residue at 105°C to constant weight, and leave no residue beyond 15mg(1.0%).
impurity
take 250g of this product, tile, observe with the naked eye or magnifying glass (5~10 times), pick out the impurities, and the impurities shall not exceed 1.0%.
acid-insoluble ash
The residue left under the item of ash is added to the Crucible with 25ml of 3mol/L hydrochloric acid solution, boiled for 5 minutes, filtered with gray-free filter paper, and the residue in the Crucible is washed with water on the filter paper, the filter residue and filter paper are transferred to the same crucible, heated slowly, and burned to constant weight according to the method under ash content. The remaining acid-insoluble ash shall not exceed 0.5%.
loss on drying
take this product (if it is strip, it should be cut and crushed), dry at 105°C for 5 hours, and lose no more than 20.0% of weight (General rule 0831).
ash
take this product about l .O g, place the Crucible into a crucible which is pre-baked to constant weight, weigh precisely, slowly burn until complete carbonization, and gradually raise the temperature to 650°C ± 2 5C, so as to completely Ash and keep the weight constant, no ash left over 5 .0%.
Heavy metals
take 0. 50g of this product, check according to law (General Rule 0 8 2 1 second law), containing heavy metals shall not exceed 40 parts per million.
arsenic salt
take this product l.O g, plus sulfuric acid 5M l fully wet (can increase the amount of sulfuric acid plus appropriate, but not more than 10ml), slow heating, control the heating temperature does not exceed 120X:, 30% hydrogen peroxide solution was added dropwise carefully, heating was stopped, mixed evenly by shaking in several times, heated again after the reaction was calm, and the above operation was repeated to keep the amount of hydrogen peroxide in a slightly excessive state, when the mixture turns brown or black, add a small amount of 30% hydrogen peroxide solution, continue to digest and gradually warm up until the disulfide oxide is completely removed and the solution becomes colorless or light yellow, add 10ml of water slowly, mix well, continue heating to remove thick smoke, repeat several times to remove all hydrogen peroxide; Cool, add 10ml of water, rinse the edge and inner wall of the container with water to make 35ml. Take the standard arsenic solution of 3 .0M l with the same method, inspection according to law (General Principles 0822 second law), should comply with the provisions (0.0003%).
microbial limit
taking this product for inspection according to law (General rules 1105 and 1106), the total number of aerobic bacteria per l g of test product shall not exceed lOOcfu, and the total number of mold and yeast shall not exceed lOOcfu, E. Coli should not be detected.
Last Update：2022-01-01 11:26:08
MACCONKEY AGAR - Category
Authoritative Data Verified Data
pharmaceutical excipients, suspending agents and release blockers.
Last Update：2022-01-01 11:26:08
MACCONKEY AGAR - Storage
Authoritative Data Verified Data
sealed storage.
Last Update：2022-01-01 11:26:08
MACCONKEY AGAR - Reference Information
| FEMA | 2012 | AGAR (GELIDIUM SPP.) |
| pH range of acid-base indicator discoloration | 5 - 7 |
| EPA chemical substance information | information provided by: ofmpeb.epa.gov (external link) |
| Introduction | the cold day (cold day powder) has been widely accepted and loved by people since the Nara period in Japan, it has been widely consumed as a dish. And to the Jianghan period, people will be the rest of the extra powder to the cold outdoor, frozen into dry goods, it is said that this is the origin of the cold. After trying to cook, the unique odor of the powder disappeared and turned into a transparent solid. Because it is rich in dietary fiber, it is also popular as a healthy food for weight loss. |
| properties | Agar is translucent white to light yellow film bands or fragments, particles and powder odorless or slightly special odor, the taste was sticky and smooth. |
| Use | agarose powder can be used for soft candy. Ice cream. Jelly. Ham. Gel powder. Lunch meat. Sausage. Tomato sauce. Coconut sauce. Dried cheese, etc. The amount of Agar is generally between 0.3 and 1.5%. 1. Grain orange beverage-Agar as a suspending agent. Its use concentration of 0.01-0.05%. Can make the particles suspended evenly. 2. Juice gummy -- the usage amount of Agar is about 2.5%. And the liquid of glucose. The gummy made of white sugar and so on. Its transparency and taste are far better than other gummy. 3. Canned meat. Meat products-use 0.2-0.5% Agar to form a gel that effectively binds minced meat. 4. Eight-baby porridge. The bird's nest of Tremella fuciformis. Soup food -- use 0.3-0.5% Agar as thickener. Stabilizers. 5. Cold food-first Agar as emulsion stabilizer and thickener, Agar has a strong gelling ability, and dextrin or sucrose when the gel strength increased. China's provisions can be used for all kinds of food, according to the production needs of the appropriate amount of use. thickener; Stabilizer; Emulsifier; Gelling agent. Commonly used in candy, sheep soup, western point, pie, ice cream, yogurt, cool drinks, dairy products, etc. In beer production, it can be used as a curing agent for copper, and the protein and tannin in the coagulation are removed after precipitation. Qiong refers to adhesives, thickeners and capsules that can be used as food thickeners, silk sizing agents, laxatives, and drugs, and can also be used as bacterial culture media, Immobilized enzyme carriers, inclusion of bacterial materials and media for electrophoresis. It can also be used for the filtration separation of Virus, subcellular particles and macromolecules, and the observation of serum antigens or antibodies. ADI (daily intake allowed) does not require special provisions. used for the preparation of bacterial culture medium, also used as a stabilizer of colored material suspension Agar because of its special gelling properties, especially significant stability, hysteresis and hysteresis, and easy to absorb water, has a special stability effect; Has been widely used in food, medicine, chemical, textile, national defense and other fields. In the food industry, it has excellent functions of extenders, thickeners, emulsifiers, gelling agents, stabilizers, excipients, suspending agents and water retention agents, and can be used to produce: Crystal soft candy, shaped soft candy, aquatic products, canned meat, fruit juice beverage, fruit beverage, rice wine beverage, dairy beverage, fine product, dairy cake |
| Use range | all kinds of food, GMP. Cheese 8; Confection and frosting 2.0%, soft candy 1.2%; Other food 0.25%. |
| identification test | take 1g of sample, add 65ml of water, boil 10 min, stir constantly, after making up with hot distilled water, it is allowed to cool to 32~39 °c, that is, it is condensed into a translucent and elastic gel, which is heated to 85 °c to start melting. The pieces of the sample were taken out, immersed in a 0.02mol/L iodine solution, and after a few minutes, stained in a brown-black color, taken out, and immersed in water to give a gradual purple color. Take 4ml of 0.5% sample solution, add 0.5ml of hydrochloric acid, heat for 30min on water bath, add 1.5ml of sodium hydroxide solution (TS-224), and 6ml of copper tartrate alkaline solution (TS-80), heat on water bath, A red precipitate should be produced. Hydrogel-forming ability a 1.0% sample solution was prepared in a flask with boiling water, and the flask was placed in water at 30 °c for 15min. A hard, durable gel should be formed. The flask was kept in water at 70 °c for 1H and the gel should not melt. Precipitation with ammonium sulfate solution should occur when 0.5% of a 40 °c warm sample solution is added to half the volume of a 40%, 40 °c warm ammonium sulfate solution. According to this, it can be distinguished from alginic acids, gum arabic, gum India, gum Platanus, pectin and tragacanth. Formation of precipitates in the case of lead acetate solution precipitation should occur when 0.5% of the warm sample solution is added to the 1/5 volume of the basic lead acetate solution (TS-129). This test can distinguish Agar from methyl cellulose. |
| content analysis | (minimum gel concentration) diluted to 0.15%, 0.20%, the second sample solution was placed in each glass tube having a length of 0.25% and an inner diameter of, respectively, and both ends were plugged. Cool at 20-25 °c for 1H. The cylindrical gel in the glass tube is then poured onto a horizontal surface, and the lowest concentration of the gel that is not broken in 5 to 30s under natural gravity is the lowest gel concentration of the sample. |
| toxicity | ADI is not restrictive (FAO/wHO,2001). GRAS(FDA,§ 184.1115,2000). LD5011g/kg (rat, oral);16g/kg (mouse, oral). |
| usage limit | GB 2760-96: all kinds of food, GMP. FAO/WHO(1984,g/kg): processing cheese manufacturing 8; Cooked ham, cooked pig leg meat, ready-to-eat broth, soup, GMP; Sardines and its products, mackerel and canned fish 20 (only in filled soup); Cream 5 (only for pasteurized cream or ultra-high temperature cream and Disinfection cream); after fermentation by heat treatment of flavored yogurt and its products 5000 mg/kg; Cold drink 10. FDA,§ 184.1115(2000): baked goods and mixed powders, 0.8%; Confection and frosting, 2.0%; Gummy, 1.2%; Other foodstuffs, 0.25%. FEMA(mg/kg): soft drinks 420~1000; Cold drinks 130~1000; Baked goods 490; Coating material 300~3000; Alcoholic beverages 200; Protein 2000. |
| production method | Agar is a viscous substance that constitutes the cell wall of red algae such as croaker and Gracilaria. When making the strip Agar, remove impurities by soaking in water, heat and hydrolyze with sulfuric acid or acetic acid at 120 ℃, about 0.1MPa (gauge pressure), pH 3.5-4.5, the hydrolysate is filtered and purified, cooled and solidified at 15-20 °c, and the gel is cut and dried at 0-10 °c. In the production of powdered Agar, the gel is cut, frozen at 13 ° C., separated, dissolved, and adjusted to a concentration of 6-7% with water. The gel is obtained by spray drying at 85 ° C. In order to improve the performance of the product, it can be treated with alkali solution to remove part of the sulfate group to increase the content of 3,6-depoly-l-galactose. Product Specification: GB1975-80. From the red algae, Stone cauliflower (Gelidium amansii L.), gracilaria con ("ervoides") and other related algae are pretreated with alkali solution, washed with water to remove alkali, boiled with weak acid solution, or extracted in an autoclave, filtered to remove slag, solidified, dehydrated, it was dried and pulverized. Or the gel obtained by filtration is frozen, thawed and dehydrated and dried. In general, it is obtained from the extraction of red algae such as stone cauliflower, coryneopsis, silk algae, and small stone cauliflower. Firstly, Stone cauliflower is soaked in water to remove impurities, and then sulfuric acid or acetic acid is soaked and hydrolyzed at 120 ℃, 1MPa and pH 3.5~4.5, after filtration and purification, the hydrolysate is cooled and solidified to form a gel at 15 to 20 ° C., and then cut into strips and dried at 0 to 10 ° C. To obtain strip Agar. For example, after cutting the agar gel, it is frozen at -13 ° C., and it is prepared into 6% ~ 7% glue solution with water, and spray dried at 85 ° C. To obtain powder Agar. |
| category | toxic substances |
| toxicity grade | low toxicity |
| Acute toxicity | oral-rat LD50:11000 mg/kg; Oral-mouse LD50: 16000 mg/kg |
| flammability hazard characteristics | flammability; Combustion-induced emission smoke |
| storage and transportation characteristics | The warehouse is ventilated and dried at low temperature; Stored separately from food raw materials |
| fire extinguishing agent | dry powder, foam, sand, carbon dioxide, water mist |
| toxic substance data | information provided by: pubchem.ncbi.nlm.nih.gov (external link) |
Last Update：2024-04-09 19:05:15
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